Metastasis of Human Melanoma Component of Complement, Is Involved in Tumorigenicity and A Cysteine Proteinase, Which Cleaves Human C3, the Third
نویسندگان
چکیده
The DM-4 humanmelanomacell line, which is highlymetastaticin nudemice,expresses a C3-cleavingactivitythatproteolyzes labeledaswell asunlabeledhumanC3. ThisC3-cleavingactivityisa cysteineproteinase characterized by a Mr 41,000. The p41 proteinase shares antigenic deter minants with murine p39 procathepsin-L and human procathepsin-L. Preincubation of DM4 cells with anti.p39 F(ab')2 induced up to 45% decreasein their complementresistance.Pretreatmentof DM-4 cellswith anti-p39Ab stronglyinhibitedtheir tumorigenicityand significantiyde creasedtheir metastaticpotential in nudemice.Thus, the p41C3.cleaving proteinasecontributes to tumongenicity and metastasisof human mela momaDM-4 cells.
منابع مشابه
Procathepsin-L, a Proteinase that Cleaves Human C3 (the Third Component of Complement), Confers High Tumorigenic and Metastatic Properties to Human Melanoma Cells1
We previously demonstrated that highly metastatic human melanoma cells secrete a 41 kDa proteinase that cleaves C3, the third component of complement, and shares antigenic determinants with procathepsin-L. Thus, we herein transfected the nonmetastatic DX-3 melanoma cells with the procathepsin-L cDNA. Three clones expressing and secreting high levels of procathepsin-L were selected. Conditioned ...
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We previously demonstrated that highly metastatic human melanoma cells secrete a 41 kDa proteinase that cleaves C3, the third component of complement, and shares antigenic determinants with procathepsin-L. Thus, we herein transfected the nonmetastatic DX-3 melanoma cells with the procathepsin-L cDNA. Three clones expressing and secreting high levels of procathepsin-L were selected. Conditioned ...
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